Specimen Collection: Have blood drawn at a hospital laboratory. I will write a lab draw order for you. The Fed Ex pickup will be handled by the draw station. Make sure to arrive before about
Test Description: The Spectracell test is done by extracting the lymphocytes from the blood sample. The the same number of lymphocytes is dispensed into 34 reaction wells.
In well #1 the lymphocytes are suspended in a solution with a nutrient broth with all the test nutrients at a concentration insufficient to fully nourish the cells under the high metabolic load of rapid multiplication for 5 days.
The sample is then stimulated to rapidly reproduce by a chemical called a mitogen (hemagglutinin). The cells are allowed to duplicate for the next 5 days. At which point, the number of lymphocytes is counted in Well #1, and that number is used as a reference number for all the other tests.
Reaction well #2-34 each have samples of lymphocytes, but with a single nutrient removed from that nutrient broth. These cells are likewise stimulated to divide by the added mitogen, and allowed to divide for the 5 day period.
At the end of the 5 days, the cells in each of the nutrient-deficient reaction vessels are counted and compared to the number in the nutrient sufficient sample. There is an optimum number of cell replications for each nutrient-deficient well.
For those cultures which duplicate at a rate less than the optimum compared to the nutrient-sufficient culture, the results are tagged as insufficient and that nutrient is recommended for supplementation.